FIG 3.

Effects of SQ109 on trypomastigotes and red blood cells. (A) Effects of SQ109 on LLC-MK₂ culture-derived trypomastigotes. Activity was evaluated after a 24-h treatment with SQ109 at 37°C. *, statistically significant at a P value of <0.001. (B) Hemolysis of human red blood cells treated with SQ109. A suspension of fresh defibrinated human blood was treated with SQ109 (10 to 80 μM) and incubated at 37°C and 5% CO₂ for 24 h, and the absorbance at 540 nm was read. Amphotericin B (AM-B) was used as the hemolytic reference drug and Triton X-100 as the positive control. All experiments were conducted in triplicate, 3 times.