TABLE 1.

Oligonucleotides used in this study

Oligonucleotide	Procedure	Sequence (5′ to 3′)
acrA_2F	PCR	CGTGTTGCCGCCATTGAGACTT
acrA_2R	PCR	GGGAACACAGCGCGTAAGGTCA
acrB_2F	PCR	GTACTTGCCATTGGCCTTTT
acrB_2R	PCR	AGGGCTTCATGACCTACACC
oprM_2F	PCR	CTGGATTTATGGGGCAAAGT
oprM_2R	PCR	GGCATTTTGAATGGCTTTTT
16S RNA_F	PCR	AAGGTTTGATC(AC)TGG(CT)TCAG
16S RNA_R	PCR	CTTTACGCCCA(AG)T(AG)A(AT)TCCG
acrA_F	Cloning	GCTGGTCATTGGGCTGAT
acrA_R	Cloning	GTTTCGTCAGCAGCGTTCAT
acrB_F	Cloning	ATCCTATCAGCCGTGTGGAG
acrB_R	Cloning	ACACCATCGAAGACACACCA
oprM_F	Cloning	TGCACGTATCTTGACGCTCT
oprM_R	Cloning	TGGGATTTTTCGTCATCCAT
acrA_F_SYBR green	SYBR green	CTCGAAACTGTGCCAGTGAT
acrA_R_SYBR green	SYBR green	ATCAATAATGCCCGTCACCT
acrB_F_SYBR green	SYBR green	TCGCTTGAGCAACAAAAATC
acrB_R_SYBR green	SYBR green	TACAGTGCTGCCAAACACAA
oprM_F_SYBR green	SYBR green	GCCAAGTCTACAAGCAGCAA
oprM_R_SYBR green	SYBR green	ACCAATCAGCAGCTGTAACG
16S RNA _F_SYBR green	SYBR green	CAATGGGCGAAAGCCTGAT
16S RNA _R_SYBR green	SYBR green	GTGCTTTACAACCAAAAGGCCT