Fig. 5.
Influence of peptide-virosome association on in vitro dendritic cell uptake and in vivo T-cell responses. M158–66-FITC peptide uptake by human immature DCs was determined by flow cytometry (a). Either PBS, peptide-loaded virosomes (P-V), peptide mixed with empty virosomes (P+V mix) or free peptide (P) were incubated at 37°C with immature DCs for 4, 24 and 48 h. Data represents mean ± SD (n = 3) performed with DCs obtained from three different donors. HLA-A2.1 transgenic C57BL/6 mice were immunized twice with aforementioned formulations adjuvanted with CpG. Peptide-specific CD8+ T-cell responses were determined in ex vivo stimulated splenocytes by flow cytometry (b) and ELISPOT (c). Data represents mean ± SD (n = 3) and is representative of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.