Effects of TMB-8,4-aminopyridine and nifedipine on movement index of colonic longitudinal smooth muscle strips in rats. A: After treatment with 8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride (TMB-8), 4-aminopyridine (4-AP) and nifedipine, the movement index (MI) of colonic longitudinal smooth muscle strips (CLSMs) in the TongXie-YaoFang (TXYF) formula group was lower than that in the normal group (n = 8, P < 0.05); B: After treatment with TMB-8, MI of rat CLSMs in the TXYF formula group was lower than that in the normal group. aP < 0.05 vs normal group.