Figure 1.

(A). Domain architecture of PtMCM. (B). SDS-PAGE (12%) analysis of purified recombinant PtMCM: Coomassie stain. Mr – molecular weight markers. Arrowhead indicates PtMCM. (C). Gel filtration analysis of PtMCM. Numbers with arrowheads indicate molecular weights and retention volumes of protein calibration markers. SDS-PAGE analysis of fractions matching the retention volumes are shown (full-length gel can be seen on-line in Supplementary Fig. S1A). (D). Western blot analysis of recombinant MCM protein with mouse anti-MCM antibodies (1:1000 dilution; full-length blot can be seen on-line in Supplementary Fig. S1B). (E). Western blot analysis of Picrophilus torridus extracts (6.5 × 10⁷ cell equivalents) with anti-MCM antibodies (1:1000 dilution). Coomassie stain shows the input extracts (1.6 × 10⁷ cell equivalents). Input extracts for Coomassie stain and for Western Blot were resolved on the same gel, the gel cut in half, one-half used for Western and other half stained with Coomassie.