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. 2015 Mar 12;5:9038. doi: 10.1038/srep09038

Figure 2. Microscopy features of the CFBE cell lines expressing mCherry-Flag-wt-CFTR (a–d) or mCherry-Flag-F508del-CFTR (e–h).

Figure 2

Cells were grown in the presence of 1 μg/ml Dox so as to induce CFTR expression. DIC bright field images (a, e) showing an overall cell morphology reminiscent of CFTR localization, especially for F508del-CFTR, where its ER accumulation is visible. Widefield fluorescence images show triply labelled unpermeabilized cells. Nuclei are stained with hoechst 33342 (b, f), mCherry fluorescence is proportional to the total amount of expressed CFTR (c, g) and Alexa Fluor® 647 (immuno)fluorescence is proportional to the amount of Flag tags exposed extracellularly (i.e. CFTR molecules present at the PM) (d, h). Fluorescence images were obtained under equivalent conditions for both cell lines. Scale bar = 20 μm.