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. 2015 Mar 12;5:9038. doi: 10.1038/srep09038

Figure 6. Small molecule screening assay with CFBE cells expressing the mCherry–Flag-F508del-CFTR construct.

Figure 6

Cells were treated with DMSO, VX-809 (3 μM), C3 (10 μM), C4 (10 μM), C18 (10 μM) or VX-809 plus C4. Immunofluorescence images of mCherry-CFTR and extracellularly exposed Flag-tags (a) were quantified so as to determine total CFTR expression, PM CFTR amounts and traffic efficiency (total/PM CFTR) versus the DMSO negative control (b). From all tested compounds VX-809, used in isolation, was the most effective rescuing F508del-CFTR traffic. The deviation score is calculated according to Formula 2. Scale bar = 50 μm. Data is presented as median ± SEM. Horizontal dashed lines represent the threshold defined in Formula 2. *p < 0.01 (t-test).