Fig. 5.

SERCA2a activity. a The enzyme-linked NADH assay for measuring SERCA2a activity and calculating ∆pK_Ca. SERCA2a ATPase activity is coupled to phosphorylation of pyruvate by pyruvate kinase. NADH oxidation is coupled to dehydrogenation of pyruvate to form lactate. The oxidation of NADH is measured by absorbance at 280 nm. b ∆pK_Ca of U-PLB (U), P16-PLB (P16), P17-PLB (P17), and 2P-PLB (2P) with the reference point of pK_Ca for SERCA2a alone. The figure was reproduced from Ablorh et al. 2014 with permission from the American Society of Biological Chemistry and Molecular Biology