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. 2015 Mar 12;5:9047. doi: 10.1038/srep09047

Figure 3. Location and qualitative expression of IκB-α, p-IκB-α and NF-κB p65 in murine colon epithelia.

Figure 3

IF was performed using specific antibodies to label target proteins. NF-κB p65 was limited to the epithelial cytoplasm in the normal colon, and nuclear localization can be observed after DSS-induced colitis. However, a significant reduction in p65 nuclear translocation was seen in 2-Cl-IB-MECA-treated mice. There was a tendency for decreased IκB-α and increased p-IκB-α expression in the cytoplasm after DSS colitis, which was inhibited in the 2-Cl-IB-MECA-treated group. These results suggested that 2-Cl-IB-MECA inhibited DSS-induced phosphorylation of IκB-α and the nuclear translocation of NF-κB p65 in colonic epithelia.