Figure 2. MEN-lgl^KD tumours show a response to hypoxic stress.

(a) Imaginal wing discs from w; Rpl27A¹, en-Gal4, UAS-RFP/+; UAS-lgl-RNAi/+ individuals show a hypoxic P compartment as indicated by positivity to the hypoxia probe pimonidazole (green). (b, c) Imaginal wing discs from w; Rpl27A¹, en-Gal4, UAS-GFP/+; UAS-lgl-RNAi/+ individuals. (b) Sima/HIF1α accumulation is visible in the P compartment (magnification). (c): imaginal wing discs from w; Rpl27A¹, en-Gal4, UAS-RFP/+; UAS-lgl-RNAi/bnl-LacZ individuals show that the bnl/FGF promoter is ectopically activated in many tumour cells (arrow); the physiological source of Bnl/FGF is indicated by the arrowhead. The A/P border is dotted in green and disc contour is outlined in white in a and c. The genotype of the P compartment is indicated in each image.