Figure 5.

Nampt functional activity on NF-κB gene expression. FLAG-Nampt plasmid as well as NF-κB luciferase reporter vector was transiently transfected into Huh-7 cells. After one-day incubation, the cells were treated with 10 nM FK866 for 1 hour before H₂O₂ treatment for 6 hours. Cells were changed into normal medium for 16–18 hours incubation. The lysates were harvested and measured their luciferase activity normalized with CMV-drive Renilla luciferase activity in each transfection. The experiment was performed in triplicate. § P value < 0.1 (6 versus 8); ^** P value < 0.05 (1 versus 3); ^*** P value < 0.01 (1 versus 2, 1 versus 4, 1 versus 5); ^### P value < 0.01 (5 versus 7).