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. 2015 Feb 1;13:47. doi: 10.1186/s12967-015-0410-7

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© Ouyang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotypes of HLA-DR ⁻ CD33 ⁺ CD11b ⁺ MDSCs in CRC patients. HLA-DR⁻CD33⁺CD11b⁺ cells were gated as MDSCs from either PBMCs (A) or tumor tissues (B) from CRC patients. The phenotypes of the MDSCs were analyzed with flow cytometry using multiple anti-human mAbs against CD14, CD15, CD66b, CD39, CD73, CXCR4, CD117, CD34, ARG1, INOS, PD-L1 and DCFDA as indicated, and the grey curve represents autofluorescence as a negative control. Representative histograms are shown.