Table 1.
Primers used for construction of the H. pylori ggt isogenic mutant strains
| Primer name | Sequence (5′- 3′) | Primer use |
|---|---|---|
| pBlue linear Fwd 1 | GGGGATCCACTAGTTCTAGAGCG | Linearization of plasmid |
| pBlue linear Rev1 | CGGGCTGCAGGAATTCGATATCAAG | Linearization of plasmid |
| HpGGT-flank_fusion1F | CTTGATATCGAATTCCTGCAGCCCGTAACCGGTAAAATCAACACGGACGC | Amplification H. pylori ggt and partial up- and downstream flanking genes |
| HpGGT-flank_fusion1R | CGCTCTAGAACTAGTGGATCCCCGCGCTCTTATAAAAAGAAGCCGC | Amplification H. pylori ggt and partial up- and downstream flanking genes |
| pBluelinear_Hpggtflank1F | CCAAGGAAAGAATTTTAATCCTATTTAG | Linearization of the recombinant plasmid |
| pBluelinear_Hpggtflank1R | CTGTTTTCCTTTCAATCAACAATAATC | Linearization of the recombinant plasmid |
| Hpkana_fusion_1F | ATTATTGTTGATTGAAAGGAAAACAGATGATTGAACAAGATGGATTGC | Amplification kanamycin resistance gene |
| Hpkana_fusion_1R | CTAAATAGGATTAAAATTCTTTCCTTGGTCAGAAGAACTCGTCAAGAAG | Amplification kanamycin resistance gene |
| T7 prom3 | TAATACGACTCACTATAGGG | Sequencing |
| M13R | CAGGAAACAGCTATGAC | Sequencing |