Figure 3.

Silencing SCRN1 expression inhibited cell invasion. (a) Transwell invasion assay was performed to measure cell invasion in RKO and HCT cells. Number of invaded cells was significantly reduced due to SCRN1 silence. Data was presented as mean ± SD, P < 0.01 in both cases. (b) Real-time PCR revealed that no significant difference in MMP-2/9 mRNA expression was observed between negative control and SCNR1 knockdown cells. Data was presented as mean ± SD, P > 0.05 in both cases. (c) Elisa assay revealed that SCRN1 knockdown reduced MMP-2 protein secretion in both RKO and HCT116 cells. Results are presented as means ± SD and P < 0.05 in both cases.