Figure 4.

Generation and differentiation of neurospheres from human ADSCs. (a) Human ADSCs were planted in a low-attachment culture dish in neurosphere culture medium at high density, and neurospheres gradually became organized within a few hours. (b) The number of neurospheres increased over 24 h. (c) After 72 h, the large sphere clumps became dark in the center. (d) After 5 days, the spheres became even darker. (e) Neurospheres were seeded onto poly-d-lysine culture dishes and were cultured in the neurogenic medium and, 2 days later, the cell mass adhered to and spread across the growth surface. (f) At 14 days, most of the cells in neurospheres showed progressively extended long processes similar to typical neural shapes. Phase contrast images at 100x magnification are shown.