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. 2015 Mar 7;11:50. doi: 10.1186/s12917-015-0350-8

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© Pasternak et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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OVA-specific cytokine production by PBMCs from piglets gavaged with OVA subunit vaccine then i.p. immunized with OVA/IFA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. IL-10 production was measured from PBMCs obtained four weeks post i.p. immunization. PBMCs were re-stimulated with OVA or media ex vivo. After 72 hours, the supernatants were collected and measured by BioPlex assay. Each data point represents an individual animal and median values are indicated by horizontal lines.