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. 2015 Mar 3;16:10. doi: 10.1186/s12865-015-0077-0

Figure 1.

Figure 1

CD62p expression of platelets in static incubation system. A: Flow cytometry analysis of the effect of pCRP and mCRP on the CD62p expression of platelets incubated statically with ANCA-induced netting neutrophils. ADP at a concentration of 100 μmol/L was used as a positive control. Results are expressed as the percentages of CD62p positive platelets. Four tubes of each group were analyzed and averaged. B-G: Representative images of the flow cytometry analysis of the percentages of platelets expressing CD62p. B: PRP were incubated with ANCA-induced netting neutrophils alone. C: PRP were incubated with ANCA-induced netting neutrophils pretreated with DNase I (0.1 U/μL). D: PRP were incubated with pCRP (60 μg/mL) alone. E: PRP were incubated with mCRP (60 μg/mL) alone. F: PRP were incubated with ANCA-induced netting neutrophils and mCRP (60 μg/mL). G: PRP were incubated with ANCA-induced netting neutrophils, mCRP (60 μg/mL) and DNase I (0.1 U/μL).