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. 2015 Mar 3;16:10. doi: 10.1186/s12865-015-0077-0

Figure 5.

Figure 5

Influence of the mCRP deposited during perfusion experiments on platelets activation. A: Representative images of activated platelets (CD62p positive) after immobilized ANCA-induced netting neutrophils were perfused with pCRP-containing PRP or mCRP-containing PRP. The concentration of pCRP or mCRP was 60 μg/mL. Activated platelets were visualized using FITC conjugated anti-CD62P mAb (green). Scale bar: 50 μm. B: Calculated levels of the deposited CD62p positive platelets after immobilized ANCA-induced netting neutrophils were perfused with PRP which did not contain pCRP or mCRP, with pCRP-containing PRP or with mCRP-containing PRP. Results were expressed as activated platelets/visual field. Five visual fields of each group were analyzed.