FIGURE 1.

Design of the haploid inducer screen. Seeds of Col-0 strains containing the glabra1 mutation are mutagenized to obtain M1 plants which are self-fertilized to produce M2 plants. These plants, either in M1 or M2, are crossed with an apt mutant (A). The two mutations, apt and glabra1 are recessive and confer resistance to 2FA and absence of trichomes, respectively (B). In F1, if gynogenesis or androgenesis haploids appear they would lack APT or GLABRA dominant allele, respectively, and be detected because of the corresponding phenotype.