FIGURE 3.

Region-specific expression levels of established microglial markers. No significant differences were found in AIF1 (A), CD11b (B) and TLR2 (C) mRNA levels between the different brain regions. For CD68 (D) and IL-1β (E), a significantly higher expression was present in the OB compared to other brain regions (CD68: OB vs. Str, HC, AM ^∗p < 0.05; IL-1β: OB vs. Str ^∗p < 0.05), and a significantly higher expression of CD68 (d) in Str compared to AM was found (^#p < 0.02). Significantly higher TNF mRNA level (F) was measured in the SN relative to the HC (^∗p < 0.05), a significantly higher CCR2 mRNA level (G) in SN compared to OB (^∗p < 0.05), significantly lower P2X₇R mRNA level (H) in SN compared to Str, HC, and AM (^∗p < 0.05), and significantly more P2X₇R expression (H) in the HC compared to OB (^#p < 0.05). (I) No GFAP expression was observed, indicating that the sorted microglia were not contaminated by astrocytes.