Table 1.
Primer sequences used for q-PCR analysis.
| Rat genes | Alignment sequence | Forward (5′→3′) | Reverse (5′→3′) | bp |
|---|---|---|---|---|
| GAPDH | NM_017008.3 | GAACATCATCCCTGCATCCA | GCCAGTGAGCTTCCCGTTCA | 79 |
| GFAP | NM_017009.2 | CAGACTTTCTCCAACCTCCAG | CTCCTGCTTCGAGTCCTTAATG | 138 |
| TNFα | NM_012675.3 | CCACACCGTCAGCCGATT | TCCTTAGGGCAAGGGCTCTT | 81 |
| TLR2 | NM_198769.2 | GAGCATCGGCTGGAGGTCT | CTGGAGCTGCCATCACACAC | 160 |
| ITGAM | NM_012711.1 | TTATTGGGGTGGGAAACGCCT | CTGGAGCTGGTTCCGAATGGT | 137 |
| AIF1 | NM_017196.3 | GCCTCATCGTCATCTCCCCA | AGGAAGTGCTTGTTGATCCCA | 142 |
| IL-1β | NM_031512.2 | AAAGAAGAAGATGGAAAAGCGGTT | GGGAACTGTGCAGACTCAAACTC | 81 |
| CD68 | NM_001031638.1 | CTCATCATTGGCCTGGTCCT | GTTGATTGTCGTCTGCGGG | 80 |
| NOS2 | NM_012611.3 | AACTTGAGTGAGGAGCAGGTTGA | CGCACCGAAGATATCCTCATGA | 81 |
| MSR1 | NM_0011939.1 | AAAGGCAGGGAGGTCAGGATTTC | CCGCTACCATCAACCAGTCG | 89 |
| P2X 7R | NM_001038839.1 | ACCCTCAGTGTTCCATCTTCCG | TTCCTCCCTGAACTGCCACC | 84 |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFAP, glial fibrillary acidic protein; TNFα, tumor necrosis factor alpha; TLR2, toll-like receptor 2; ITGAM, integrin, alpha M (CD11b); AIF1, allograft inflammatory factor 1 (Iba-1); IL-1β, interleukin-1 beta; CD68, cluster of differentiation 68; NOS2, nitric oxide synthase 2; MSR1, macrophage scavenger receptor 1; P2X7R, P2X purinoreceptor 7 bp, amplicon size base pair.