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. 2015 Mar 12;6:205. doi: 10.3389/fmicb.2015.00205

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Copyright © 2015 Silva-Dias, Miranda, Branco, Monteiro-Soares, Pina-Vaz and Rodrigues.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biofilm formation by different Candida species. Biofilm was quantified colorimetrically by two different methodologies: (A) Crystal violet assay, that measures biofilm total biomass and (B) XTT assay, which measures biofilm metabolic activity. Error bars represent the standard deviation among results for different isolates. Each isolate was tested for its ability to form biofilm at least 3 times. Asterisks represent cases were a statistically significant difference in the values at 24 and 48 h were observed. Due to asymmetric distribution and sample size, in several comparisons non-parametric tests were used.