Table I.
Oligonucleotide microarray analysisa
| Endothelial gene expression profile of Duffy transfectants |
|---|
| Following GRO-α/CXCL1 stimulation; no significant changes |
| Following TNF-α stimulation |
| Up-regulated genes: first 10 of 42 up-regulated genes |
| 1. VCAM-1 |
| 2. Human IFN-γ treatment inducible mRNA |
| 3. Human JE gene encoding a monocyte secretory protein (MCP-1) |
| 4. Diubiquitin |
| 5. Human endothelial leukocyte adhesion molecule-1 (E-selectin) |
| 6. ICAM-1 |
| 7. Human TNF-inducible early response gene |
| 8. Homo sapiens mRNA for KIAA106 protein |
| 9. Human adhesion molecule ninjurin mRNA |
| 10. Human B94 protein mRNA |
| Down-regulated genes: first 10 of 142 down-regulated genes |
| 1. Human signal transducing adaptor molecule 2A (STAM2) mRNA |
| 2. Human SNF-1-like protein kinase mRNAb |
| 3. Human homolog of yeast mutL (hPMS1) gene |
| 4. H. sapiens mRNA for vascular Rab-GAP/TBC-containing proteinb |
| 5. H. sapiens RP58 gene |
| 6. AI961743:wt66f12.x1 H. sapiens cDNA |
| 7. Human mRNA for brain-derived neurotrophic factor |
| 8. Human TRF-1-interacting ankyrin-related ADP-ribose polymerase mRNAb |
| 9. Human myosin H chain 12 (MYO5A) mRNA |
| 10. Human msg1-related gene mRNA |
a
The gene expression profile was examined in endothelial Duffy transfectants stimulated with 20 nM GRO-α/CXCL1 or 10 ng/mL TNF-α for 4 h using oligonucleotide microarrays. No genes were found to be significantly up-regulated or down-regulated in the Duffy+ cells following GRO-α stimulation. In Duffy+ cells stimulated with TNF-α, 42 genes were up-regulated and 142 genes were down-regulated compared with unstimulated cells (the first 10 in each group are shown for simplicity). The results were similar in two independent experiments.
b
SNF, sucrose nonfermenting protein; GAP/TBC, GTPase-activating protein/Tre/Bub2/Cdc16 domain; TRF, telomeric repeat binding factor; msg, missing.