Table 6.

qRT-PCR confirmation data

Group	CT	CT mean	CTU6 mean	∆C T	NC ∆C T	∆∆C T	2−∆∆CT
hsa-miR-1179
AS	29.807	30.090	22.855	7.235	7.790	−0.555	1.469
AS	29.937	30.090	22.855	7.235	7.790	−0.555	1.469
AS	30.527	30.090	22.855	7.235	7.790	−0.555	1.469
NC	31.033	30.833	23.043	7.790	7.790	0.000	1.000
NC	30.743	30.833	23.043	7.790	7.790	0.000	1.000
NC	30.723	30.833	23.043	7.790	7.790	0.000	1.000
hsa-miR-4461
AS	26.902	27.317	22.855	4.461	11.719	−7.258	153.045
AS	27.758	27.317	22.855	4.461	11.719	−7.258	153.045
AS	27.290	27.317	22.855	4.461	11.719	−7.258	153.045
NC	34.766	34.762	23.043	11.719	11.719	0.000	1.000
NC	34.910	34.762	23.043	11.719	11.719	0.000	1.000
NC	34.611	34.762	23.043	11.719	11.719	0.000	1.000
hsa-miR-4651
AS	22.085	22.050	22.855	−0.805	0.965	−1.770	3.411
AS	22.182	22.050	22.855	−0.805	0.965	−1.770	3.411
AS	21.882	22.050	22.855	−0.805	0.965	−1.770	3.411
NC	25.018	24.008	23.043	0.965	0.965	0.000	1.000
NC	24.549	24.008	23.043	0.965	0.965	0.000	1.000
NC	24.756	24.008	23.043	0.965	0.965	0.000	1.000
Novel-miR-22
AS	21.190	21.139	22.855	−1.717	1.839	−3.556	11.760
AS	21.069	21.139	22.855	−1.717	1.839	−3.556	11.760
AS	21.157	21.139	22.855	−1.717	1.839	−3.556	11.760
NC	24.813	24.882	23.043	1.839	1.839	0.000	1.000
NC	24.925	24.882	23.043	1.839	1.839	0.000	1.000
NC	24.910	24.882	23.043	1.839	1.839	0.000	1.000
Novel-miR-81
AS	22.867	22.866	22.855	0.011	6.399	−6.388	83.754
AS	22.703	22.866	22.855	0.011	6.399	−6.388	83.754
AS	23.029	22.866	22.855	0.011	6.399	−6.388	83.754
NC	29.244	29.442	23.043	6.399	6.399	0.000	1.000
NC	29.778	29.442	23.043	6.399	6.399	0.000	1.000
NC	29.305	29.442	23.043	6.399	6.399	0.000	1.000
Novel-miR-185
AS	31.310	31.149	22.855	8.294	10.029	−1.735	3.329
AS	31.173	31.149	22.855	8.294	10.029	−1.735	3.329
AS	30.964	31.149	22.855	8.294	10.029	−1.735	3.329
NC	32.942	33.072	23.043	10.029	10.029	0.000	1.000
NC	33.463	33.072	23.043	10.029	10.029	0.000	1.000
NC	32.811	33.072	23.043	10.029	10.029	0.000	1.000

∆C _T=CT mean−CT_U6 mean; ∆∆C _T=∆C _T−NC ∆C _T