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. 2015 Mar;16(3):235–250. doi: 10.1631/jzus.B1400272

Table 6.

qRT-PCR confirmation data

Group CT CT mean CTU6 mean ∆C T NC ∆C T ∆∆C T 2−∆∆CT
hsa-miR-1179
 AS 29.807 30.090 22.855 7.235 7.790 −0.555 1.469
 AS 29.937 30.090 22.855 7.235 7.790 −0.555 1.469
 AS 30.527 30.090 22.855 7.235 7.790 −0.555 1.469
 NC 31.033 30.833 23.043 7.790 7.790 0.000 1.000
 NC 30.743 30.833 23.043 7.790 7.790 0.000 1.000
 NC 30.723 30.833 23.043 7.790 7.790 0.000 1.000
hsa-miR-4461
 AS 26.902 27.317 22.855 4.461 11.719 −7.258 153.045
 AS 27.758 27.317 22.855 4.461 11.719 −7.258 153.045
 AS 27.290 27.317 22.855 4.461 11.719 −7.258 153.045
 NC 34.766 34.762 23.043 11.719 11.719 0.000 1.000
 NC 34.910 34.762 23.043 11.719 11.719 0.000 1.000
 NC 34.611 34.762 23.043 11.719 11.719 0.000 1.000
hsa-miR-4651
 AS 22.085 22.050 22.855 −0.805 0.965 −1.770 3.411
 AS 22.182 22.050 22.855 −0.805 0.965 −1.770 3.411
 AS 21.882 22.050 22.855 −0.805 0.965 −1.770 3.411
 NC 25.018 24.008 23.043 0.965 0.965 0.000 1.000
 NC 24.549 24.008 23.043 0.965 0.965 0.000 1.000
 NC 24.756 24.008 23.043 0.965 0.965 0.000 1.000
Novel-miR-22
 AS 21.190 21.139 22.855 −1.717 1.839 −3.556 11.760
 AS 21.069 21.139 22.855 −1.717 1.839 −3.556 11.760
 AS 21.157 21.139 22.855 −1.717 1.839 −3.556 11.760
 NC 24.813 24.882 23.043 1.839 1.839 0.000 1.000
 NC 24.925 24.882 23.043 1.839 1.839 0.000 1.000
 NC 24.910 24.882 23.043 1.839 1.839 0.000 1.000
Novel-miR-81
 AS 22.867 22.866 22.855 0.011 6.399 −6.388 83.754
 AS 22.703 22.866 22.855 0.011 6.399 −6.388 83.754
 AS 23.029 22.866 22.855 0.011 6.399 −6.388 83.754
 NC 29.244 29.442 23.043 6.399 6.399 0.000 1.000
 NC 29.778 29.442 23.043 6.399 6.399 0.000 1.000
 NC 29.305 29.442 23.043 6.399 6.399 0.000 1.000
Novel-miR-185
 AS 31.310 31.149 22.855 8.294 10.029 −1.735 3.329
 AS 31.173 31.149 22.855 8.294 10.029 −1.735 3.329
 AS 30.964 31.149 22.855 8.294 10.029 −1.735 3.329
 NC 32.942 33.072 23.043 10.029 10.029 0.000 1.000
 NC 33.463 33.072 23.043 10.029 10.029 0.000 1.000
 NC 32.811 33.072 23.043 10.029 10.029 0.000 1.000

∆C T=CT mean−CTU6 mean; ∆∆C T=∆C T−NC ∆C T