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. 2015 Mar;16(3):235–250. doi: 10.1631/jzus.B1400272

Table S1.

qRT-PCR primers used in the validation assays

Primer name Sequences 5' to 3' Melting temperature, Tm (°C)
mir-4461-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACTATGGC
mir-4461-F TATGTACGTAGTCTAGGCC 55
mir-1179-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACACCAAC
mir-1179-F AAGCATTCTTTCATTGGTTGG 55
mir-4651-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACCCCACC
mir-4651-F CGGCGACGGCGGGGT 55
Novel-mir-81-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACGCCACG
Novel-mir-81-F GGGGATGGGGAGGGGC 55
Novel-mir-202-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACAAGTGT
Novel-mir-202-F TATGAATGTTGATGGGTACAC 55
Novel-mir-185-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACAGAGGA
Novel-mir-185-F TGGGCGGCCACTTGACAT 57
miRNA-R GTCGTATCCAGTGCGTGTC
U6-RT GTCGTATCCAGTGCGTGTCGTGGAGTCGG
CAATTGCACTGGATACGACAAAATATG
U6-F GATTAGCATGGCCCCTGC 55