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. 2015 Mar 12;13(3):e1002109. doi: 10.1371/journal.pbio.1002109

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© 2015 Gallie et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Transposon insertions in SBW25 genes pflu3658–78 (filled triangles = precise insertion site identified, and unfilled = precise site unknown; numbers relate to S1 Table strain names). See S1 Fig for reactions catalysed by enzymes disrupted by transposon insertion. (B) Comparison of the SBW25 and E. coli K-12 colanic acid biosynthetic loci. The SBW25 locus is predicted to contain five genes involved in CAP precursor biosynthesis (yellow), wcaJ (red), four glycosyl transferases (purple), two acetyl transferases (blue), five genes involved in polymerisation and transport (green), and four genes of unknown function (white). Homologous gene products (connected by grey lines) were identified with Basic Local Alignment Search Tool protein (BLASTP, a tool used to search a query protein against a protein database).