Table 1. Parameters derived from iMSD analysis.
TRPV1 pool | D (μm2/s) | α | d (nm) | τb (s) | #cells | A (%) | |
TRPV1-C | ∼ 0 | - | 360±40 | 16.4±4.0 | 22 | 68±21 | Basal |
* | * | * | * | 8 | 69±14 | +NDZ | |
* | * | * | 2.9±2.1 | 10 | 88±08 | +SMase | |
TRPV1-T | (9.0±2.6) ∙10−3 | 1.5±0.3 | 860±300 | »60 s | 22 | 19±16 | Basal |
- | - | - | - | - | 0 | +NDZ | |
- | - | - | - | - | 0 | +SMase | |
TRPV1-I | 0.22±0.02 | 1 | - | - | 22 | 13±09 | Basal |
0.99±0.18 | * | - | - | 8 | 31±15 | +NDZ | |
0.10±0.04 | * | - | - | 10 | 12±07 | +SMase |
The rows indicate the different dynamic populations of TRPV1. For each population we describe three conditions: basal, upon nocodazole (+NDZ) or sphingomyelinase (+SMase) administration. For the columns, we report dynamic parameters from left to right: Diffusion coefficient (D), anomalous diffusion exponent (α), diameter of diffusing species (d), binding time (τb) and amplitude of populations (A). The values are expressed as Mean±Standard Error.
* Values keeping equal at basal condition (obtained by STICS-FRET analysis) during the fitting.
- Not Determinate.