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. 2015 Mar 12;10(3):e0116321. doi: 10.1371/journal.pone.0116321

Fig 3. Binding capacity of miR-128 with individual putative targets determined by luciferase report assay.

Fig 3

(A) A set of tests included 3’UTR-target-luci vector alone, EGFP-miR-128 vector alone, and combination of 3’UTR-target-luci vector and EGFP-miR-128 vector. The luciferase activity was detected by Xenogen IVIS instrument. The EGFP background in the assay was subtracted from the luciferase activities. The results represented the mean ± SD for 2 independent experiments with triplicates and P<0.05. (B) Levels of BMI-1 and BAX mRNA expression in cultured cell lines were determined using QRT-PCR.