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. 2015 Jan 30;95(5):859–874. doi: 10.1111/mmi.12909

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© 2014 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Genome-wide distribution of recombination events in AS25-3.A. High-quality SNPs of the parental strains P2niaD18 and AS25 were compared with the progeny AS25-3. For a genome-wide scale, sliding window analysis with 10 kb windows were performed to display the origin of the sequence. Regions clearly assigned to P2niaD18 are blue; regions inherited from AS25 are red.B. To validate selected crossing over sites in detail, 500 bp sliding window analysis and Sanger resequencing was performed. As an example, a recombination site within a 7 kb region, containing 27 SNPs, from chromosome II was verified (position is indicated by triangle). The predicted region of recombination, framed in yellow, is located between the two adjacent SNPs that are differently inherited.