Fig 6. Tool compounds increase activity of human GBA1 when added into mouse brain lysates.
A) Compounds 40 & 43 robustly increase activity of human purified GBA1 when added to mouse brain lysates (Mouse+rhGBA) while they do not substantially affect mouse GBA1 (Mouse), as measured by turnover of 4MUG substrate. Vehicle treated samples were set as 100%. A representative experiment is shown. B) Greater activation of human recombinant protein in mouse lysates is seen without sodium taurocholate. Activity of human recombinant protein in mouse lysates was obtained by subtracting values from a parallel experiment with mouse brain lysates but without added human recombinant protein. C) Table depicting EC50 values and % signal for purified human protein added to mouse brain lysates (three independent experiments). Mean ± SEM is shown.