Fig 2. Screening procedures for non-SMC subunit mutants of S. pombe condensin.

A. Procedures consist of eight steps, starting from construction of integration plasmids and ending with verification of the cnd mutant phenotype produced by chromosomal re-integration (see text). B. Twenty-one temperature- and/or DNA damage-sensitive cnd mutant strains containing various single amino acid substitutions were obtained. Their classification according to phenotype is shown in the table (see text). Ts^-, temperature sensitive; Ds^-, damage sensitive; Ts^-Ds^-, temperature and damage sensitive.