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. 2015 Mar 12;10(3):e0119347. doi: 10.1371/journal.pone.0119347

Fig 4. Phenotypes of cytological, drug, and synthetic genetic interaction phenotypes of mutants.

Fig 4

A. DAPI-stained S. pombe wild-type (WT) and mutants cells cultured at 26°C and 36°C for 2.5 h. Cells displaying severe defects in chromosome segregation were observed in mutant cells at 36°C. More than 100 mitotic cells for each mutant strain were counted. Almost all (95∼100%) mitotic mutant cells observed after 2.5 hrs at 36°C showed severe defects of chromosome segregation as shown here. Mutant cells (cnd1-G984R) cultured in 2 mM HU for 2 h, which showed severe segregation defects at 26°C, are also shown (see text). Bars are 10 μm. B and C. In YPD liquid medium at the restrictive temperature, 36°C, ts mutants ceased to divide within 4 h and lost viability. Asynchronous cultures of WT and ts mutants (cnd1-H1133P, cnd2-A708V and cnd3-L269P) in the YPD liquid medium were shifted from 26°C to 36°C. Cell density (per mL) was determined and viability was measured after a temperature shift to 36°C. D. S. pombe wild-type (WT), and cnd1 and cnd3 mutant cells were spotted on solid agar plates in the presence or absence of HU, CPT, and UV irradiation. E. Synthetic genetic interactions between non-SMC ts mutants and SMC mutants (cut14-208, cut3-477 and cut14-Y1). The left table summarizes all genetic interactions examined. The right panel shows the synthetic defect between cnd2-A708V and 2 SMC mutants (cut14-208 and cut3-477). See text.