FIGURE 6:

Ryanodine receptor inhibition prevents axonal BDNF transport defects. (A, B) Representative images of Ret3 and RyR immunocytochemistry. ER was detected in the dendrites and axons of tau^−/− neurons. Axons were distinguished by standard morphological criteria and by the absence of MAP2. (C) Inhibition of RyRs prevented AβO-induced transport defects independent of tau. Dantrolene treatment maintained normal anterograde and retrograde flux in the presence of AβOs. Graphs show means ± SEM. A minimum of 15 cells from three different cultures were analyzed per condition; ***p < 0.001 relative to controls. Tau^+/+ transport data are presented in Supplemental Table S3. Complete statistical evaluation is presented in Supplemental Table S3. Scale bar, 25 μm.