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. 2015 Mar 15;26(6):1106–1118. doi: 10.1091/mbc.E14-03-0812

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© 2015 Vilas, Krishnan, Loganathan, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — CAII expression does not increase water flux through AQP2 expressed in HEK293 cells. Aquaporin-mediated (AQP1 and AQP2) water transport activity was measured by confocal fluorescence microscopy of transfected HEK293 cells as per Figure 4. (A) Rate of fluorescence change corrected for activity of vector-transfected cells and normalized to AQP1 or AQP2 cell surface expression. Cell surface expression was determined by biotinylation and is presented in Supplemental Figure S7. Number sign (#) represents a statistically significant difference (p < 0.05) compared with AQP1; n > 3 per condition. (B) Immunoblots demonstrating total cellular expression of AQP1, AQP2, CAII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).