FIGURE 6:

CAII interacts with the acidic clusters in the C-terminus of AQP1 by peptide overlay assay. Fifteen–amino acid peptides corresponding to overlapping progressively more C-terminal sequences in AQP1 were spotted onto cellulose membranes and visualized with ultraviolet fluorescence (tryptophan fluorescence). The exact peptide sequence is detailed above the blot. Recombinant CAII or albumin was incubated with the membrane before a primary anti-CAII antibody, followed by the appropriate secondary antibody and then visualization. Representative overlays from three separate experiments.