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. 2015 Mar 15;26(6):1106–1118. doi: 10.1091/mbc.E14-03-0812

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© 2015 Vilas, Krishnan, Loganathan, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — AQP1 and CAII are in close proximity. (A) The CAB mutants generated. The CAB1 motif is in blue, and the CABII motif is in red. (B) Immunoblots of cell lysates from transfections used in the proximity ligation assays. The primary antibody used is depicted under the blot, and the cDNA transfected is displayed in bold above the blot. GAPDH was probed as a loading control. (C) Cells transfected with the indicated cDNAs were processed in a proximity ligation assay, using an anti-CAII antibody in each case, along with anti-AE1 (AE1), anti-AQP1 (AQP1, CAB1AQP1, CABIIAQP1, CABI/IIAQP1), or anti-hCNT3 (hCNT3).