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. 2015 Mar 15;26(6):1119–1128. doi: 10.1091/mbc.E14-08-1281

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© 2015 Bean et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Vrl1 is a new yeast VPS9-domain protein. (A) Schematic of yeast VPS9 domain–containing proteins and human VARP based on Superfamily (Gough et al., 2001) and ClustalW alignments. (B) Yeast were spotted in 10× dilution series and grown 2 d at 37°C to assess temperature sensitivity of the indicated strains. VRL1 was expressed from its endogenous promoter. (C) A colony overlay assay was used to assess carboxypeptidase Y (CPY) secretion. Cells spotted in 10× dilution series were overlaid with nitrocellulose and incubated for 16 h. The nitrocellulose was then immunoblotted with anti-CPY antibodies.