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. 2015 Mar 15;26(6):1119–1128. doi: 10.1091/mbc.E14-08-1281

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© 2015 Bean et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — The yeast VPS9-domain proteins Muk1, Vps9, and Vrl1 localize to endosomes. (A) MUK1pr-GFP-Muk1, ADH1pr-GFP-Vps9, and ADH1pr-GFP-Vrl1 localize to Snf7-RFP–marked late endosomes by fluorescence microscopy. (B) Fluorescence microscopy of ADH1pr-GFP-Muk1 with Snf7-RFP, the lipophilic dye FM4-64, and the tagged retromer subunit RFP-Vps26. (C) Schematic of Muk1 truncations used in D, showing conserved C-terminal motifs identified by alignments (purple). (D) Fluorescence microscopy of full-length or mutated ADH1pr-GFP-Muk1. (E) Deletion of VPS35 does not disrupt the endosomal localization of overexpressed GFP-Vps9 or GFP-Muk1. Scale bars, 2 μm; OE, overexpressed.