TABLE 1:
Antibodies used in this work.
| Antibody | Species/type | Clone | Application/dilution | Source |
|---|---|---|---|---|
| α-Stim2 | Rabbit, pAb | NA | WB 1:500 | Cell Signaling (Danvers, MA), 4917S |
| α-Stim1 | Rabbit, pAb | NA | WB 1:500 | ProSci (Loveland, CO), 4119 |
| α-GluA1-NT | Mouse, mAb | RH95 | WB 1:1000, IP 1:10, ICC 1:500 | Millipore, MAB2263 |
| α-pGluA1 Ser-845 | Mouse, mAb | EPR 2148 | WB 1:1000 | Millipore, 04-1073 |
| α-pGluA1 Ser-831 | Mouse, mAb | N453 | WB 1:1000 | Millipore, 04-823 |
| α-GluA2 | Rabbit, pAb | WB 1:1000 | Synaptic Systems (Goettingen, Germany), 182 103 | |
| α-GluN1 | Mouse, mAb | M68 | WB 1:1000 | Synaptic Systems, 114 011 |
| α-CaMKII | Rabbit, pAb | NA | WB 1:1000 | Cell Signaling, 3362 |
| α-syntaxin | Mouse, mAb | HPC1 | WB 1:20,000 | Sigma-Aldrich, S0664 |
| α-VAMP2 | Mouse, mAb | SP10 | WB 1:1000, ICC 1:1000 | Covance (Princeton, NJ), MMS-616R |
| α-Homer | Rabbit, pAb | NA | ICC 1:1000 | Synaptic Systems, 160003 |
| α-PSD95 | Mouse, mAb | 70-18 | WB 1:1000 | Millipore, MAB1596 |
| α-PKA-R1-α/β | Rabbit, pAb | NA | WB 1:1000 | Cell Signaling, 3927 |
| α-PKA-C-α | Rabbit, mAb | EP2102Y | WB 1:1000 | Abgent (San Diego, CA), AJ1612a |
| α-PKA-C-α | Mouse, mAb | 5B | ICC 1:500 | BD Transduction Laboratories (San Jose, CA), 610980 |
| α-AKAP150 | Goat, pAb | NA | IP 1:10 | Santa Cruz Biotechnology, sc-6445 |
| α-AKAP150 | Rabbit, pAb | NA | WB 1:1000 | Millipore, 07-210 |
| α-calnexin | Rabbit, pAb | NA | 1:2000 | Abcam (Cambridge, UK), 22595 |
| α-actin | Mouse, mAb | AC15 | WB 1:20,000 | Sigma-Aldrich, A1978 |
| α-GAPDH | Mouse, mAb | 6C5 | WB 1:20,000 | Millipore, MAB374 |
| α−NR1 | Mouse, mAb | NA | WB 1:1000 | Millipore, 05-432 |
| α−NR2A | Rabbit, pAb | NA | WB 1:1000, IP 1:10 | Millipore, 07-632 |
| α−Orai1 | Rabbit, pAb | NA | WB 1:1000 | ProScience, 4281 |
| α-rIgG HRP | Goat | NA | WB 1:10,000 | Jackson, 111-001-003 |
| α-mIgG HRP | Goat | NA | WB 1:10,000 | Jackson, 115-001-003 |
| Alexa Fluor 488 Anti-mIgG | Goat | NA | ICC 1:1000 | Invitrogen, A110011 |
| Alexa Fluor 568 anti-mIgG | Goat | NA | ICC 1:1000 | Invitrogen, A11004 |
| Alexa Fluor 488 anti-rIgG | Goat | NA | ICC 1:1000 | Invitrogen, A11008 |
| Alexa Fluor 568 anti-mIgG | Goat | NA | ICC 1:1000 | Invitrogen, A11011 |
| Alexa Fluor 633 anti-mIgG | Goat | NA | ICC 1:500 | Invitrogen, A21050 |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ICC, immunocytochemistry; mAb, monoclonal antibody; pAb, polyclonal antibody; WB, Western blot.