FIGURE 3:

Interaction between Fam13a and 14-3-3. (A) GST pull down, showing that Fam13a interacted with bacterially expressed GST–14-3-3η and GST–14-3-3ε but not GST. (B) Western blot, showing coimmunoprecipitation of myc-Fam13a and FLAG–14-3-3η. FLAG–14-3-3η was detected when myc-Fam13a was immunoprecipitated (middle). Conversely, myc-Fam13a was detected when FLAG-14-3-3η was immunoprecipitated (right). (C) GST pull-down assay, showing that GST–14-3-3 interacts with the full-length Fam13a and some Fam13a deletion constructs (1–609, 1–513, 1–393, and 1–341). GST-14-3-3 failed to pull down 1–158. (D) Western blot, showing that truncation of residues 315–329 decreased the binding between GST–14-3-3 and Fam13a.