FIGURE 8:

Generation and analysis of Fam13a-knockout mice. (A) RT-PCR, showing the expression of FAM13A in adult mouse tissues. (B) Schematic diagram showing the knockout strategy. Arrowheads indicate primers used for genotyping. KO-F and GT-B were used to detect the knockout allele (KO). WT-F and GT-B were used to detect the wild-type allele (WT). (C) Genotyping PCR showing the genotype of offspring from intercrossing between Fam13a^+/− mice. (D) RT-PCR, showing the expression of Fam13a in wild-type, heterozygous, and knockout animals. (E) Sequence of the PCR product amplified from a Fam13a^−/−-knockout animal, showing deletion of exon 5. (F) Histological analysis of the large airways (left) and alveoli (right) in a wild-type mouse and a FAM13A-knockout mouse. (G) Representative real-time RT-PCR result, showing the levels of axin2 mRNA in control and Fam13a^−/− lungs. (H) Representative real-time RT-PCR result, showing that knockdown of Fam13a reduced the level of axin2 mRNA in A549 cells.