Fig. 4.
In the absence of p23 HDAC1 promotes the DNA binding activity of HSF1. (a) Aliquots of in vitro translated HSF1 were mock treated or immunodepleted of p23 (P23:IP ✓), incubated at 43°C for 30 min with radiolabeled HSE, in the presence or absence of HDAC1 with or without supplementation of p23 to normal reticulolysate levels. (b) Aliquots of in vitro translated HSF1 were mock treated or immunodepleted of p23 (P23:IP ✓), incubated at 43°C for 30 min with radiolabeled HSE, in the presence or absence of wild type HDAC1 or the deacetylase point mutant H141A [43]. The reactions were resolved by native gel electrophoresis and the protein-DNA complexes were visualized using a PhosphorImager.