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M distribution for the twenty partially purified human milk HA samples after fractionation by IEX, and analysis by competitive ELSA assays. The mass ratio of the high M HA eluted at 0.800 M was scaled to 1/10 for improved visualization of the low M HA fractions. The average M (ca. 440 kDa) for the 0.800 M HA fraction from milk was separately determined by agarose gel electrophoresis.
