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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anal Biochem. 2015 Jan 9;474:78–88. doi: 10.1016/j.ab.2014.12.020

Fig 7.

Fig 7

M distribution for the 0.800 M HA fraction from human milk sample No. 14 by 1% agarose gel electrophoresis. Lane 1:Hiladder, containing HA molecular mass markers 1510, 1180, 966, 667, 495 kDa. Lane 2: Loladder and Megaladder containing HA molecular mass markers 4570, 3050, 1520, 495, 310, 214, 110, and 30 kDa. Lane 3:ca. 2.5 μgHA eluted with 0.800 M NaCl solution after 0.360, 0.425 and 0.460 M NaCl stepwise elution. The HA amount is estimated from the ELSA data. Lane 4: The same sample as in Lane 3 after specific hyaluronidase digestion. The bands lower than 100 kDa are impurities and don't contain any HA.