. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Anal Biochem. 2015 Jan 9;474:78–88. doi: 10.1016/j.ab.2014.12.020

Table 1. HA Sample Fractionation by IEX (7 fraction method and 4 fraction method).

IEX Fraction (M NaCl)	HA average M (kDa)	HA M range (kDa) (width at half height)
0.300	7±1	5±1 to 9±2
0.330	11±1	9±1 to 14±2
0.360	16±2	13±2 to 20±3
0.400	25±2	19±2 to 33±3
0.416	38±3	32±2 to 47±4
0.440	61±10	43±4 to 74±12
0.800	^*150 (>80)	79±16 and above

0.360	12	7 to 21
0.425	35	22 to 56
0.460	80	57 to 114
0.800	^*155 (>80)	108 and above

data obtained from the results of 3% agarose gel electrophoresis.