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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anal Biochem. 2015 Jan 10;474:28–34. doi: 10.1016/j.ab.2015.01.001

Fig 1.

Fig 1

(A) Derivatization of DON in 3N HCl plus n-butanol followed by LC-MS analysis – DON was heated at 60°C for 30 minutes in n-butanol containing 3N HCl to derivatize the molecule into a stable quantifiable analyte of molecular mass [M+H]+ 218.0942. (B) The high resolution mass and isotopic abundance were used to generate the molecular formula C10H16ClNO2. The observed isotopic abundance closely matched the predicted isotopic abundance of the proposed chemical structure incorporating a chlorine atom. This is apparent from the 3:1 ratio of [M] (218.0942) and [M+2] (220.0912). (C) UPLC trace of derivatized DON. Derivatized DON was injected on an Agilent 1290 LC equipped with a C18 column and detected with an Agilent 6520 QTOF mass spectrometer.