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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anal Biochem. 2015 Jan 10;474:28–34. doi: 10.1016/j.ab.2015.01.001

Fig 3.

Fig 3

Tandem mass spectrometry (MS/MS) of derivatized DON – (A) Collision induced dissociation of DON after derivatization with acidified butanol – DON was derivatized with n-butanol containing 3N HCl and analyzed by LC-MS/MS. The resulting product ions of 162.03 and 116.03 match the loss of the butyl ester and the radical formed after the loss of the entire carboxyl-ester moiety. These product ions are consistent with the expected DON derivative structure. (B) Collision induced dissociation of DON after derivatization with 3N HCl – DON was derivatized with 3N HCl without butanol and analyzed by LC-MS/MS. The resulting product ion of 116.03 is consistent with the expected DON derivative structure in the absence of butanol.