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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anal Biochem. 2015 Jan 10;474:28–34. doi: 10.1016/j.ab.2015.01.001

Fig 4.

Fig 4

(A) Standard curve of DON in plasma after derivatization with acidified butanol and LC-MS analysis – DON was spiked into untreated mouse plasma to generate standards at various concentrations. DON derivatization was carried out with n-butanol containing 3N HCl. After centrifugation to separate denatured proteins, supernatant was incubated at 60°C for 30 minutes. Derivatized DON was detected by LC-MS. Standards in the 30 nM to 100 μM range were used to generate a standard curve. (B) Standard curve of DON in brain after derivatization with acidified butanol and LC-MS analysis – DON was spiked into untreated mouse brain to generate standards at various concentrations. DON derivatization was carried out with n-butanol containing 3N HCl. After centrifugation to separate denatured proteins, supernatant was incubated at 60°C for 30 minutes. Derivatized DON was detected by LC-MS. Standards in the 30 nM to 100 μM range were used to generate a standard curve.