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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Anal Biochem. 2015 Jan 10;474:28–34. doi: 10.1016/j.ab.2015.01.001

Fig 6.

Fig 6

DON concentration in plasma from DON- treated mice using underivatized and derivatization protocols – Mouse plasma samples were obtained 15 min after DON administration (1.6 mg/kg, i.v.). LC-MS bioanalysis of underivatized DON from plasma: DON was extracted from plasma with methanol, dried and resuspended in H2O and separated in a Hypercarb column (methods). LC-MS bioanalysis of derivatized DON from plasma: DON was derivatized in butanol containing 3N HCl for 30 min at 60 °C, dried, reconstituted in 30% acetonitrile and separated by RPC (methods). Analytes eluting after each chromatographic separation were detected by QTOF MS. N= 3 for each treatment. Error bars correspond to ± S.D.